Our latest paper, Internal guide RNA interactions interfere with Cas9-mediated cleavage shows that when using CRISPR/Cas9, guide RNAs with regions of self-complementarity can inhibit the effectiveness of Cas9 cleavage. We have added the findings of this paper as an option in CHOPCHOP so that users can avoid internal guide RNA interactions.
Congratulations to the first author, postdoc Summer!
