A monumental effort by Yinan and coauthors for their exciting work “Whole-embryo Spatial Transcriptomics at Subcellular Resolution from Gastrulation to Organogenesis” now out on BioRxiv! Here, they introduce a new system whole-embryo imaging platform using multiplexed error-robust fluorescent in-situ hybridization (weMERFISH). Using this method hey spatiality quantified 495 genes in early zebrafish embryos. Moreover, they could use the signature of these genes to infer the full transcriptome and chromatin accessibility of the cells using data from the accompanying paper from Jialin and co-authors (here). This data is browsable online! Check it out here.
Read the paper here. This work was possible due to great collaborations with the labs of Bogdan Bintu (UCSD), Guoqiang Yu (Tsinghua University), Susan Mango (Biozentrum) and Ahilya Sawh (University of Toronto).