Our latest paper "Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPR–Cas9 barcodes by scGESTALT" was published in Nature Protocols.
In this study, Bushra and Jamie provide a detailed protocol for using scGESTALT to reconstruct cell lineages in zebrafish embryos. They provide details for (i) generating transgenic zebrafish; (ii) performing multi-timepoint barcode editing; (iii) building scRNA-seq libraries from brain tissue; and (iv) concurrently amplifying lineage barcodes from captured single cells.
Congratulations Bushra and Jamie!
