The latest paper from our lab has been published in PLOS One: Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
This paper outlines protocols for cloning-free generation of guide RNAs and generation of Cas9 protein, and describes sequence rules that improve guide RNA activity.
Congratulations to the first author, postdoc James Gagnon!
